Coding

Part:BBa_K977001:Design

Designed by: Eric Kessler   Group: iGEM13_BV_CAPS_Kansas   (2013-06-20)


Yeast Pyruvate Kinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1405
    Illegal XbaI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1405
    Illegal BglII site found at 515
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1405
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1405
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site-directed mutagenesis used to eliminate two illegal restriction sites within the gene.


Source

Dr. Fenton

References